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The Ru-xylA segments were recovered from pXIP1/2 and pXIδ double-digested with EcoRI/SphI.
The four genes in the PPP segments were recovered from pJPPP3/4 digested with SmiI.
Extended fragments of novel hantavirus S, M, and L genome segments were recovered from an arboreal African rodent.
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Hantavirus S-segment sequences were recovered from these animals (corresponding hantavirus strains were designated ShenyangRn20, ShenyangRn32, ShenyangRn74, ShenyangRn75, ShenyangRn111, ShenyangRn127, ShenyangRn131, ShenyangRn144, ShenyangRn167, ShenyangRn180, ShenyangRn183, and ShenyangAa13).
Hantavirus S-segment sequences were recovered from these animals (corresponding hantavirus strains were designated ShenyangRn-LAC-4, ShenyangRn-LAC-28, ShenyangRn-LAC-41, and ShenyangRn-LAC-137).
Partial S-segment sequences were recovered from 6 patient serum samples, 19 laboratory rats (designated ShenyangW–, Figure 2); 15 wild Norway rats, and 1 striped field mouse trapped in the outbreak region.
Tissues from 25 virus-infected voles were taken for genetic analysis, and partial sequences of PUUV genome S, medium (M), and large (L) segments (≈12% of the total virus genome) were recovered from them.
Isolates were recovered from clinical specimens from 1985 to 2013.
No Phytophthora spp. were recovered from either the bark or sapwood core sections from segments inoculated with zoospores, mycelium or oospore suspensions.
Two firearms were recovered from the home.
Bacteria were recovered from all teeth examined.
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