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Corresponding segments of tissue from each animal were fixed in 10% formalin or 4% paraformaldehyde (PFA) for histology, or frozen for RNA extraction.
This includes whole segments of tissue without GFP label.
The ultrahigh-speed imaging system enables the acquisition of very large data sets which cover long segments of tissue with dense spatial sampling.
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For immunostaining of cross sections, segments of tissue-engineered vessels were embedded in optimal cutting temperature compound (OCT, Tissue-Tek/Somagen, Edmonton, Alberta, Canada) and frozen at −80°C.
This can happen to people with asthma and other chronic lung diseases: a weakened segment of tissue gives way, and air moves into the space between the lung and the chest wall.
After a day, the researchers fashioned a 3-dimensional segment of tissue made of collagen and seeded it with the myoblasts.
Figure 3 shows haematoxylin and eosin and trefoil factor 3 staining of the Cytosponge specimen from a representative segment of tissue from a patient with Barrett's oesophagus.
The tissue that was sent to the local pathologist revealed segments of pituitary tissue, partly infiltrated by lymphocytes plasma cells and fibrous (Fig. 3a, b).
In the segments of hepatic tissue, no metastatic adenocarcinoma was found.
Segments of lung tissue were fixed in 10% buffered formalin (Sigma-Aldrich) and paraffin-embedded.
The prepared longitudinally and transversely cut segments of biological tissues were trimmed using a Reichert UM-03 ultramicrotome glass knife (Puzyr' et al. 1998; Floriani 2005).
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