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Cleavage site mutants were produced by replacing segments of the expression constructs with synthetic gene fragments containing substitutions.
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The corresponding segment of the expression vector was replaced with the restriction fragment containing the expected mutation.
These conotoxin families contribute to 0.3% of total conotoxin reads in segment P and PC, but make up 5.2% of segment DC and 58.4% of segment D. Analyses of the expression profiles of subclasses and individual conotoxins reveal additional patterns of differential expression.
In the SV segments, immunohistochemical analysis of the expression of the MMP-2, TIMP-2, and -3 was performed.
Instead, a clear gap is seen between the expression of en in these two segments and the expression of col covering the pmd/md boundary.
Flies deficient for collier (col) function lack ectodermal structures of the intercalary segment, and the expression of segment defining genes like engrailed and wingless is disturbed [ 34].
Abd-B seems to play a similar role in the ABLK neurons of the A8 segment, repressing the expression of the neuropeptide Lk.
It is then argued that chain entanglements result in the value of the number of segments N in the expression f= 3kTx/N increasing with chain extension x or with increasing force f.
The expression value for each genomic segment is the mean of the expression values of the loci included in that segment.
One part of this goal lies in identifying the binding locations of transcription factors (TFs)—proteins that bind to DNA segments and regulate the expression of nearby genes.
PCR primers were designed to amplify a ≤100 nt transcript segment toward the middle of the expression peak defined by RNA-Seq.
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