Intracellular calcium measurements were conducted in the dark on middle cerebral artery segments mounted in a wire myograph placed on the stage of an inverted microscope (Leica DMIRBE, Germany).
Finally, the cochleae were micro-dissected under a microscope into apical, middle, and basal segments, and mounted on slides with Fluoromount-G mounting medium.
Artery segments were mounted on tungsten wires on a Mulvany-Halpern myograph (Danish Myo Technology, Denmark) at 37°C in PSS solution and gassed with 5% CO2 in O2.
Isolated vessel segments were mounted on a custom-built superfusion chamber and held with two blunt glass needles operated by joy-stick micromanipulators (MN-151, Narashige, Japan).
Whenever introspection is required, this newly created code segment is mounted on guest VM environment by the VMM.
For immunohistochemistry, 12 µm sections of the same spinal segment were mounted on slides, washed in PBS (5 min), treated for 30 min in PBS containing lysine (20 mM, pH 7.4) and for 20 min in 3% H2O2).
Each bronchial segment was mounted on two L-shaped metal prongs.
Each vessel segment was mounted on two tungsten wires (40 μm diameter) in the organ chamber filled with MOPS buffer.
(The same smirk was present on Wednesday, when Watters tweeted out a non-apology as the backlash against his segment mounted: "My man-on-the-street interviews are meant to be taken as tongue-in-cheek and I regret if anyone found offense," he wrote, as if the problem were that the concept of a joke had to be explained to the humorless Chinese).
Vessels segments were washed, mounted on slides and observed by confocal microscopy (LSM-510Meta, Carl Zeiss, Germany).
