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Neurosensory layers were segmented using the manual adjustment to the segmentation lines, and layer thickness was measured at six different locations within the selected scans.
Additionally, one new point was acquired by combining adaptive two concavity points together to construct a training model to fit the circle equation, which can transform the erroneous straight segmentation lines into proper curve segmentation lines.
Finally, the straight and curve segmentation lines were integrated in one clustered image to achieve the segmentation results.
Furthermore, the candidate segmentation lines can be acquired by connecting each two concavity points, and then the correctness for each candidate segmentation line was evaluated by designing four acceptance criterions.
Retinal thickness maps based on automated segmentation lines delineating internal limiting membrane and Bruch's membrane proved consistently reliable, showing focal retinal thickening in the form of 'hot spots' at sites which were suspicious for new activity on SD-OCT (Table 1).
Four stiches were placed close to marking spots and one at the border with S7 at the diaphragmatic surface along the scheduled segmentation lines with 4 0 PROLENE® (Johnson & Johnson; New Jersey, USA) and also made another marking above the nodule with silk (Fig. 2b).
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This layer includes the retinal pigment epithelium, the relatively low reflecting transition zone to the outer segments, the outer segments and the inner segmentation line at the interdigitation of outer and inner segments.
For the RPE_OScomplex, with the intraretinal segmentation line at the level of the inner and outer photoreceptor segments, the difference was 3.32 μm, corresponding to 5%%.
The segmentation line defining the upper and lower border of the RNFL was required to be on the internal limiting membrane and lower border of the RNFL.
The segmentation line that defines the upper border of the retina was required to be on the internal limiting membrane and the lower border was required to be on the lower border of the RNFL (for RNFLT) or between the inner and outer photoreceptor layer of the RNFL (for TMV).
Two independent graders manually corrected all the images; this may have resulted in some inaccuracies in segmentation line correction.
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