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In the presence of wild target DNA, the probe is extended by DNA polymerase over the free segment of target as the template.
Furthermore, in one of the techniques used in this study (INNO-LiPA HPV Genotyping), short-PCR-fragment (SPF 10) primers are employed, which amplify a 65 base pair segment of target DNA and this testing is considered to be one of the most sensitive PCR assays for the detection of HPV DNA.
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M1, M2, M5 and M6 are linked markers for negative selection of linked segments of target genes.
Selection of plants similar to the recurrent parent from BC3F1 stage was the strategy followed in the study and the graphical genotyping data supports that view as genotype SPJ53-21-77 had 97% of the recurrent parent genome having donor segments of target resistance genes xa5, xa13 and Xa21 and further no linkage drag in regions flanking Xa21, xa13 and xa5 is observed.
After the insertion of segments of target gene template DNA, the PstI digestion sites were replaced.
Unique primers were designed for ~100 bp segments of target gene transcripts using QuantPrime online software; Table 1).
The distance between a segment of the target utterance and a stored phone is calculated using Dynamic Time Warping (DTW).
After three times backcross, all the improved lines had the segment of the target gene from donors.
Second, she understands the mindset of a big segment of your target user base -– the majority, in fact, if you're in a consumer-facing business.
This system involves the use of two oligonucleotide probes which are located close to each other and are complementary to an internal segment of a target DNA of interest.
They were able to capitalize on a segment of the target audience who preferred hiring struggling college students to a large corporation.
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