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The concepts of segment insertion and subdivision (moving boundary within the segment) are adopted in this model to track the phase change point along the flow channel.
For the segment insertion and subdivision functions, four different approaches are provided in order to achieve the best accuracy and least computational effort under various flow configurations and fluid conditions.
PCR with flanking primers indicated that 14 of the other 43 Shimaa strains also contained this insertion; an equivalent cagA-cagB segment insertion was found in Venezuelan strain v225d [30].
This superficial masseter muscle segment insertion is the most posterodorsal.
The third stain (accession number: GQ845125) has a short segment insertion, with 4 methods supported.
The causative mutation for FXID have been identified by the authors [ 32] who found that the mutation consists of a 76 bp segment insertion into exon 12 in bovine chromosome 27.
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However, there are known evolutionary phenomena, such as segment insertions, translocations and reversals, which may result in a reordering or re-rooting of RNA structural elements [ 16].
The accuracy was validated by evaluating the overlap between manually segmented insertion sites on the white light color images and on the corresponding spectral unmixed autofluorescence images.
Finally, we investigated the role of charged and polar flanking sequences on H-segment insertion.
To measure the efficiency of TM-segment insertion into the inner membrane, we have taken advantage of the peculiar maturation pathway of Mgm1p, a nuclearly encoded, dynamin-like GTPase important for mitochondrial fusion, morphology, and genome integrity in Saccharomyces cerevisiae (Sesaki et al, 2003; Wong et al, 2003; Sesaki and Jensen, 2004).
In the case of sequence errors corresponding to inserted segments (internal insertions, N/C-terminal insertions), 16 of the 23 inserted segments were conserved in closely related organisms, although 5 of them had one or more stop codons.
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