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A 10 mm spinal cord segment centred on the injury site was removed and left in fixative for 2 hours at 4°C.
A 10 mm segment centred on the injury site was collected, immediately immersed into a pre-weighed tube containing PBS with protease inhibitor, weighed and kept at −20°C.
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Neuronal profiles, immunoreactive for individual cellular markers (NeuN, PGP9.5 or NSE), were counted manually in 5 µm thick paraffin sections at every 500 µm throughout the 10 mm spinal cord segments centred on the injury site.
Analysis was restricted to 43aa segments centred on amino acids encoded by the USS core that showed >50% amino acid identity across all homologues.
Segments of well-conserved H. influenzae proteins, centred on USS-encoded amino acids, were aligned with homologous protein segments from Escherichia coli, Vibrio cholerae and Pseudomonas aeruginosa, whose genomes do not contain USS-like repeats.
Segment durations were matched by omitting, or appending, overlapping windows using a Gaussian function precisely centred on each glottal pulse.
An analysis centred on the statistically interesting words furnishes important insights into the unique elements of each segment.
Mounted and centred on the billboard is the advertisement, which is printed in 10 to 14 segments.
Most conflict centred on the budget.
Particular focus centred on the Californian.
Local rivalries have also centred on drugs.
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