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Open image in new window Fig. 5 Bone segment alignment following distraction and realignment, lateral (a) and AP (b).
The reason for this is not clear as the M segment alignment and BMCMC analysis was not remarkably different from those of the other genome segments.
As expected the secondary structure of the Mal TIR domain is similar to that of the templates with the exception of a nine amino acid deletion before the start of the 4th helical segment (alignment position 114 to 122 in Figure 2).
SGSC alignment mirrors SGN neurite segment alignment.
Both habits had effects on developing dentition, most notably on upper labial segment alignment and the development of anterior open bites and posterior cross bites [ 4].
Three alignment algorithms were used: T-COFFEE, which combines information for both global and local homologies [ 34], CLUSTALW, a progressive alignment algorithm [ 35], and DIALIGN [ 36], a local segment alignment algorithm.
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Based on best blast hits of the segments against the chicken genome, we constructed 508 protein segment alignments between Alligator mississippiensis and chicken, 429 segment alignments between Chrysemys picta (a turtle) and chicken, and 138 segment alignments between Anolis smaragdinus (another lizard) and chicken.
Based on well-aligning best blast hits of protein segments against the chicken genome (protein blast e-value < 10-5), we constructed 508 protein segment alignments between Alligator mississippiensis and chicken, 429 segment alignments between Chrysemys picta (a turtle) and chicken, and 138 segment alignments between Anolis smaragdinus (another lizard) and chicken.
Tophat uses Bowtie [ 12] for alignment and is able to detect novel splice junctions by splitting reads to segments and joining segment alignments [ 11].
The gene segment alignments were manually concatenated to generate a single alignment of complete genome sequences, and duplicate genomes (having 100% nucleotide identity) were removed.
In previous work (Smith et al., 2008), we described a novel multi-segment alignment method and empirically demonstrated that it classifies and aligns our toxicogenomics data better than several competing methods, including dynamic time warping, several parametric methods (such as linear alignment) and another multi-segment method called correlation-optimized warping, or COW (Nielsen et al., 1998).
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