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The citrate-stabilized silver seeds were prepared using sodium borohydride as a reducing agent under ice-cold condition.
Organic extracts of seeds were prepared using four different solvents with increasing polarity-hexane, ethyl acetate, acetone, and ethanol.
The aqueous, methanolic, and acetonic extracts of T. ammi and F. vulgare seeds were prepared using soxhlet extraction assembly and subjected to qualitative and quantitative estimation of phytochemical constituents.
The RNA probes for the in situ mRNA hybridization analyses of ATG5, CAT, RBR and βG transcripts in developing and mature seeds were prepared using a PCR-based technique [ 49].
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Total RNA from developing seeds was prepared using Trizol reagent as described before [ 10].
Total RNA from developing seeds was prepared using Trizol reagent following the manufacturer's protocol (Invitrogen, USA).
The radioactive nanorods were prepared using a seed-mediated method that involved the addition of a suspension of Au spherical seeds to a growth solution in the presence of CTAB as a capping agent.
Semi-solid slurries were prepared using the SEED process and injected into a high pressure die casting press.
Gold nanorods were prepared using the seed-mediated growth method, and they are stabilized with cetyltrimethylammonium bromide (CTAB) in aqueous solutions.
ZnO nanoseeds on the FTO surface were prepared using an alcohothermal seeding method.
Citrate-stabilized gold nanoparticles with small size were prepared using a modified seed-mediated method reported by Frens et al. [27].
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