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Arabidopsis thaliana ecotype Columbia was obtained from Lehle Seeds (Round Rock, TX) and used throughout this study.
The pgr7 mutant was identified from fast neutron mutated M2 seeds (Ler) obtained from Lehle Seeds (Round Rock, TX).
Arabidopsis thaliana wild type cultivar Columbia (Col-0) seeds were procured from Lehle Seeds (Round Rock, TX, USA).
For the EMS mutagenesis, seeds were incubated in water containing 0.23% ethylmethane sulfonate (EMS) for 12 hours at room temperature, washed several times with water and dried (Lehle Seeds, Round Rock, USA).
Cultures of pTRV1 were mixed in a 1∶1 ratio in a 2-liter plastic container with either pTRV2-TdPDS (silencing treatment) or empty TRV2 cultures (mock control), adding 100 ul/l Silwet L-77 (Lehle Seeds, Round Rock, TX) as a surfactant.
The RIL parents Est-1 (Estland; CS6701; male) and Kend-L (Kendalville, MI USA; Lehle-WT-16-03; male) were obtained from the ABRC Arabidopsis Biological Resource Centerr, Ohio State University, OH, USA) and from Lehle Seeds (Round Rock, TX, USA), respectively.
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