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The occasional poly-embryonic seedlings (twins, etc).
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Among the 282 individuals analyzed by flow cytometry, 27 samples had nuclei with a fluorescence peak at channel position 200 (not accounting for twin seedlings), which were considered as haploids, and were confirmed by our observations of cytology and morphology.
More than 2,000 of these seedlings (including all those with twin embryos) were transferred to the nursery prior to further screening.
Here, we combined a large-scale visual survey for undefined atypical palm seedling phenotypes coupled with active selection for seeds with twin embryos to assemble a sub-population of seedlings enriched for H/DHs.
Spontaneous H/DH seedlings are often associated with aberrant germination features, such as twin embryos from the same carpel [ 20], providing a defined feature for phenotypic selection.
The first screen utilised 10,900,000 seedlings from a wide range of crosses and identified 3,854 morphological 'off-types' (H/DH candidates), of which 53 had twin embryos and 3,801 were phenotypically abnormal.
Seedlings grow.
The resulting seedlings were weak.
But — twins.
Ask twins!!!!
Not twins?
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