For the living seedlings, stem height was measured and, when the plants were harvested, root and shoot biomass was separated.
These fungi infect floral tissues as well as seedlings, stem bases and roots causing Fusarium head blight (FHB), seedling blight, crown rot and root rot, respectively [ 1, 2].
These studies are valuable but do not permit examination of the role of fire in enhancing the establishment and growth of new oak seedlings stemming from oak mast events.
Transcripts of numit rps13 and mt rps13 were observed in roots (from seedlings), stems, stigmas + styles, and ovaries.
A small fan is needed nearby to move the air, helping to prevent fungus diseases and to flex those little seedling stems, enabling them to grow stronger and thicker, just like going to the gym.
Total RNA was isolated from 2-month-old seedling stems as described above.
The pre-miRNA sequences of 5 miRNAs, pde-miR162a, pde-miR390a, pde-miR396a, pde-miR482c and pde-miR1314a could not be amplified from total RNAs of two-month-old seedling stems, although we repeated our experiments.
To verify that miRNAs can regulate their target mRNA expression in Chinese fir, we performed a modified RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) experiment, using total RNA extracted from 2-month-old seedling stems (see Methods).
Further, rhizogenesis was reported to occur in callus tissue developed from young Spathoglottis seedling stems in response to auxins and varying the nutritional contents of the culture media (Bapat and Narayanaswamy 1977).
Fig. 5 Expression patterns of TCD10 by RT-PCR analysis; a Expression of various tissues; YR, young-seedling roots; YS, young-seedling stem; YL, young-seedling leaf; SL, second leaf; FL, flag leaf at heading; PN, young panicles.
Fig. 5 Expression analysis of TCM5 by RT-PCR analysis; YR, young-seedling roots; YS, young-seedling stem; YL, young-seedling leaf; FL, flag leaf at heading; PN, young panicles.
