A 250 g sample of seedling material was homogenized in 1000 ml buffer (0.5 M sucrose, 50 mM Tris-HCl, 10 mM EDTA, 1% bovine serum albumin, 5 mmol/L β-mercaptoethanol, 1.5% polyvinyl-pyrrolidone, pH 7.5).
James Joyce, BC Timber sales, BC, Randy Moench, Colorado State University Nursery, CO and Bill Taylor, Webster Forest Nursery, WA kindly provided seedlings material for this study.
In this study, using maize seedlings as materials, effect of NaHS treatment on heat tolerance and trehalose accumulation, as well as its possible metabolic pathways were investigated, the purpose was to expound interaction between H2S and trehalose in the acquisition of heat tolerance in maize seedlings.
The Affymetrix 22K Barley1 chip [39] was used to examine the effects of short or prolonged cold on the transcriptome of barley seedlings (see materials and methods, Figure 1A).
This revealed genes whose expression levels in K+-starved seedlings were up-regulated or down-regulated more than 1.2-, 1.5-, 2.0-fold (P-value < 0.05) compared with control seedlings (see "Materials and Methods" for details).
This paper adopts medium culture method, taking "Zheng Dan 958" maize seedlings as sample materials.
For other materials, seedlings were transferred to soil and maintained under long-day conditions (16 hour light/8 hour dark) at 26 28°C in the greenhouse.
To examine the detailed gene expression changes of Populus CCCH genes under drought stresses, RT-qPCR analysis was performed on the six Populus CCCH genes using 4-month-old P. deltoides seedlings (see the materials).
The stability of transcript abundance of these genes was analysed by QRT-PCR across 15 different samples from a variety of developmental (dry seed, imbibed seed, leaf and roots from young and old plants) and stress treated tissues (shoots from cold treated and heat treated young seedlings over time; Materials and methods).
The mass screening using seedbox test in the greenhouse uses BPH nymphs with free-choice of plant materials at seedling stage and sometimes the test is extended to different stages of plant growth.
