Control seedlings (Light, n = 10; Light-Dark, n = 13; Dark, n = 10) were left in darkness and undisturbed during the testing day.
A total of 83 seedlings (Light, n = 28; Light-Dark, n = 30; Dark, n = 25) were individually trained inside a Y-maze, where the fan [F] and the blue LED light [L] were always positioned together on one of the arms of the maze (as in the [F + L] group of the first experiment).
On the testing day, experimental subgroups of seedlings (Light, n = 18; Light-Dark, n = 17; Dark, n = 15) were kept at their treatment-specific temperature cycles in the dark, while being exposed to the fan blowing for three 90-min sessions separated by a 90-min interval.
Normally, in wild-type seedlings, light inhibits gravitropism (Fankhauser and Casal 2004); thus, another feature of the cop/det/fus phenotype is the constitutive inhibition of gravitropism in the dark.
Here we examine genetic interactions between det1 and ddb1b-2 in dark-grown seedlings, light-grown seedlings and adults.
DDB1 complexes have been implicated in photomorphogenesis and other light-regulated processes, so the phenotypes of ddb1a, ddb1b-2, and the ddb1a ddb1b-2/+ and ddb1b-2 ddb1a/+ heterozygotes in dark-grown seedlings, light-grown seedlings, and adults were examined.
Augspurger, C. K. & Kelly, C. K. Pathogen mortality of tropical tree seedlings: experimental studies of the effects of dispersal distance, seedling density, and light conditions.
PORA is quickly degraded upon seedling exposure to light, while PORB continues to be expressed in mature tissues and thus is primarily responsible for continued chlorophyll production.
4°C/7 d 25°C/10 d (Xie et al. [2012]) Seedling growth 1 = dark green seedlings, 3 = light green seedlings, 5 = yellow seedlings, 7 = brown seedlings, 9 = seedlings dead.
Vegetation composition, tree seedling emergence, light availability, soil water content, and temperatures under each treatment were measured.
