When these seedlings were labelled with FM4-64 for 2 3 min, no co-labelling between FM4-64 and GFP VTI13 was observed in root hairs (Fig. 3B), indicating that GFP VTI13 did not localize within the plasma membrane.
Root samples of 6-day-old rice seedlings were labeled with 10 μM CM-H2DCF-DA (Molecular Probes, Eugene, OR, USA) or Oregon Green 488 BAPTA-1 (Molecular Probes) for 30 min to determine ROS or calcium levels, respectively, then treated with 50 ppm FA for 1 3 h.
Seedlings for metabolically labeled mass spectrometry experiments were grown in 1× M&S micronutrient solution supplemented with 1.5 mM CaCl2, 0.75 mM MgSO4, 0.625 mM KH2PO4, 2.3 mM MES salts, and 29.2 mM sucrose.
In 2001, 20,000 one-meter-square subplots were laid out in the forest, and every seedling in them was measured and labeled.
In general, asexually propagated cultivars grown from seeds produce highly variable seedling plants, and should not be labelled with, or sold under, the parent cultivar's name.
To label the cell walls, seedlings were incubated in a propidium iodide solution (1.7 mM) for 30 s before confocal imaging analysis.
Was there some warning on the plastic label that came with the seedlings — which you stupidly threw out — that you missed?
To label the carbon made by each seedling, the team briefly enclosed each plant in a plastic bag filled with carbon dioxide containing a specific carbon isotope.
Six hundred bare-rooted P. radiata seedlings and cuttings were labelled by GF type and planted in three rectangular blocks with equal numbers of each seed lot planted in each block (Additional file 1, Fig. 1b).
One of the key dynamic components of sustainability in uneven-aged forest management is the survival and development of the small trees, which are often labelled regeneration, seedlings and sapling in practical terms with variable definitions.
