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Exact(3)
5.15 μg and 4.80 μg of total RNA were extracted from anther and seedling controls, respectively.
We hypothesize that the meiosis-specific genes can be identified by comparing transcriptome profiles of meiocytes and anthers with seedling controls.
To provide a technical replicate for the seedling controls, the seedling reads were split into two unique columns based from the position of the polony (each lane on the flow cell contains two columns that are imaged - the columns were split into two separate columns and were individually normalized by total reads per million).
Similar(57)
Raw Illumina reads have been deposited into NCBI's SRA (sequence read archive) under the study title "mRNA-seq of Zea mays B73 early-prophase meiocytes, anthers, and seedling control", accession numbers SRX218264-218270.
In contrast, the steady state levels of RbR1, PCNA1, and Cyclin D3 1 (CycD3;1) in the two calli stayed unchanged relative to the seedling control.
After defining a criterion of fold change value > 2 (either up- or down-regulated) in comparison to untreated 7-days-old seedling control, a total of 46 OsPP genes were found to be differentially expressing.
Seed disinfection and seed or seedling quality controls guarantee growers on the sanitary status of their planting material.
Additionally, single or double knockout lines of RAP2.4 and RAP2.4L grew faster than wild type plants (Additional file 3), suggesting their role in seedling growth control.
Volatiles released from undamaged 10 day-old Zea mays (cultivar Delprim) seedlings (control) and seedlings treated with caterpillar oral secretion (herbivory).
Therefore, selection of pure M. boninensis seedlings, controlled crossing and ex situ conservation are all necessary to ensure propagation of the species.
To examine which of the miRNA family members are more abundantly expressed in rice seedlings, we inspected the expression profile of known miRNAs in untreated seedlings (control library) based on their sequence frequencies.
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