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While a subset of genes was continuously expressed over 3 or 5 h post-IR (K1), another subset followed a biphasic regulation vs time (K3), indicating that seedling cells still experienced differential gene cycling after IR.
The plastid defects seen in sco1-1 and sco1-4 are most pronounced in seedling cells that were derived from the embryo.
We propagated the majority of species in individual seedling cells (3.8 × 3.8 × 6 cm) in summer 2010, held overwinter in a cold-room or cold-frame, and transferred them to a warm environment on 12 January 2011.
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For example, they exhibit defective embryonic and/or seedling cell patterning, including vein patterning, are typically seedling-lethal, and cannot be rescued by exogenous application of BRs [28].
Bright fluorescence was observed in extracellular space of seedling root cells by Confocal Laser Scanning Microscopy (Figure 9).
(B ) Histogram representing microtubule severing rates of mock treated seedlings and in seedling of cells adjacent to site of ablation after 4 hours.
Seeds from 15 plants per population were germinated in petri dishes on moist filter paper and seedlings were transplanted individually into 38 ml seedling tray cells filled with sterilized mineral sand (sterilized by gamma radiation ≥ 25 kGray).
Given that brassinosteroids mainly regulate cell elongation and considering that during seedling establishment, cell expansion is the by far dominant growth mechanism, it is surprising that the authors have not considered determining hypocotyl elongation.
Kong et al. ([1999]) studied on the change of permeability and protective enzyme activity of maize seedlings cell membrane under Cd2+ stress (Kong et al. [1999]).
For Catharanthus, RNA-sequencing data is also available for different tissues, seedlings, cell suspension cultures, and hairy root cultures.
The pairs of cis-NATs were confirmed to be formed in laser microdissection-captured rice seedling leaf epidermal cells, which were developed from a single-cell tissue.
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