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After growing for 6 mth, each seedling (1 cm stem diameter) was bud grafted with the scion RRIM 600.
Three soil treatments were applied prior seedling: (1) unamended soil treated with conventional mineral fertilizer diammonium phosphate (control), (2) soil amended with 30 t ha−1 of traditional manure (cow manure), and (3) soil amended with 30 t ha−1 of palm tree compost (P-compost).
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The causes of segregation distortion have been suggested to include aneuploidy, chromosomal translocation, competition among gametes, and the inheritance of alleles affecting the viability of the zygote, embryo, or seedling [ 1, 36, 37].
The fertiliser type used in the series was 0 1 1(17%) PK and spot applied at 170 g seedling− 1 (72.3 kg ha− 1 elemental P and K, respectively).
Survival was measured as a categoric trait (i.e. live seedling = 1, dead seedling = 0).
They were grouped into five categories, < 1 cm (seedling), 1 2 cm (juvenile 1), 2 5 cm (juvenile 2), 5 10 cm (juvenile 3), and > 10 cm (adult).
It initiated the metabolic activity of the emerging seedling [56].
To mount an Arabidopsis seedling (1 2 weeks old) for fluorescence imaging, 400 μl 1x imaging solution was pipetted into the open-top chamber and an intact seedling removed from MS medium was positioned lengthwise into the imaging solution.
Plant the rooted sprig or seedling 5 centimeters deep.
CPN60 was essential for development of embryo and seedling [ 56].
Pyridoxine-β-D-glucoside was found in rice bran, callus and seedling [ 36- 38].
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