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From the desired cell density, area of the cell seeding surface, and the suspension volume of each well, we calculated and used the appropriate suspension volume to achieve the target cell density per unit area.
The cell seeding surface layer was cut and removed.
The AdMSCs formed a layer on the seeding surface of chitosan films, showing almost no cells in the core.
The inner cross-section of specimen at approximate depth of 1 mm below the seeding surface was observed for live and dead cells using a fluorescence microscope (Olympus Corp., Japan).
Fluorescence from the migrated cells was observed at an approximate depth of 1 mm below the seeding surface.
Cells from the seeding surface could migrate into the inner bulk pores via interconnected pore structures and distributed in the entire scaffold.
Similar(54)
The first involves arranging a seeded surface to offer the shadowing effect [9].
To form smaller nanohelices than those grown by self-shadowing, a seeded surface is required to tune their morphology [14].
By forming layers of textured ZnO films by ALD on a substrate, a seeded surface can be used to fabricate high-density vertical nanorod arrays.
Apparently thicknesses below 120 nm related with short ALD deposits give seeded surfaces with highly c-axis-oriented seeds whose size determines the nanorod diameter.
By cooling the substrate and controlling its spin rate, two spiral-like and one screw-like gold nanohelix arrays are grown upon the seeded surfaces.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com