The results following FNAB procedures with and without the anti-seeding device are shown in Table 2. Seeding frequencies in standard FNAB procedures at the two clinical centres did not differ significantly (Fisher's exact test, two-sided, P=0.4466).
E7 127 was chosen as it had the highest thermodynamic score of the siRNAs that were classed as having 'low-seed frequency' (Table 1).
As an additional measure of the effectiveness of our miRNA target identification, we examined miRNA seed match frequency in mRNAs associated with RISC in a miRNA dependent manner.
Seed frequencies of orchard crops, annual cereals and pulses, and wild or weedy plants are used to characterize plant utilization at different archaeological sites on the island of Cyprus, in the Rift Valley of Jordan, and in the Jabbul Plain and along the upper Euphrates River valley in Syria.
Our protocol entails a sample size (n) of 60 seeds per lot, allowing detection of transgenic seed frequencies >0.045 (i.e., >4.5%) at P<0.05 [12].
More general studies have shown that disease persistence is maximized when connectivity between patches is of intermediate intensity as there must be a balance between the asynchrony of subpopulation dynamics and the frequency of seeding events [15], [16], [17].
First, this F2 family was selected specifically because of its low rate of seed abortion (frequency of embryo-less seeds, as estimated by assessing floating in water, was lower than for other available F2s from the maritime pine breeding program, unpublished results), making it particularly suitable to genetic analysis requiring a large sample size.
Spatial patterns of seed deposition were assessed at three different levels: (1) seed deposition frequency by carnivores, with all plant species pooled; (2) seed deposition frequency from different fruit types, i.e. wild and cultivated fruits; and (3) seed deposition frequency of different plant species.
The joint distribution of seeding times and the unordered frequency spectrum are derived in File S1, section 2. Assuming that the ith haplotype establishes at t i * and has same fitness as the founding haplotype, it will also rise in frequency, albeit with a time lag t i *.
