Secondly, when dormant TRCs were seeded back to rigid plastic plate to induce their differentiation, p27 expression was remarkably decreased even in the presence of Kyn (Fig. 4g).
The cell pellet was resuspended in fresh medium with serum and a part of the cells were seeded back into the flask.
After treatment, 5 × 10 viable cells were seeded back into a 10-cm dish in DMEM/F12 with 10% FBS.
Plasmids, or the entire genome of the recipient, could then be cloned into small insert expression vectors, transformed into E. coli or other hosts, and seeded back onto media containing the study compound.
Enter seed-backed BuzzTale.
Both Eden and Notion seed-backed the UK company, before adding a further $5 million Series A in May 2011.
"I brought the seeds back from the Caribbean myself," Mr. Kandefer said.
But we weren't looking at seventh or eighth seed back".
People would bring seeds back, or physically bring plants back, or have them shipped from relatives in the Caribbean".
Dad was recycling — pots, stakes, bulbs, seeds — back when it cost 25 cents to fill the mower with gas.
