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In this study, a novel tri-culture system was developed to elicit pre-vascularization of calcium phosphate cement (CPC) scaffold in which human induced pluripotent stem cell-derived mesenchymal stem cells (hiPSMSCs) were seeded together with human umbilical vein endothelial cells (HUVECs) and pericytes.
However, if neutrophils are seeded together with DC (at 1∶1 ratio), neutrophil migration as induced by IL-8 was abolished.
PaSMC and HUVEC were seeded together, centrifuged briefly at 200 g to achieve an even distribution of cells and cultured in EGM-2 for at least 72 hrs to allow network formation.
For the primary screen, three IR macrophage clones (each expressing either YFP, DsRed, or AmCyan) were seeded together in each well and simultaneously infected with each mutant in the Mtb library.
If Ehrlichia indeed are transported through the filopodia, we reasoned that the bacterial load will decrease in the presence of cytochalasin D when both infected and uninfected DH82 cells are seeded together as the pathogen cannot be transported to the uninfected cell.
AFSC were seeded together with iron oxide particles (BioMag, 1.6 μm diameter, 1 mg/ml) and cultured for 24 h after which particle uptake was assessed.
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Recombinant Wnt5a of 200 ng/ml (R&D Systems, Minneapolis, MN, USA) was added immediately after seeding together with the FZD7 siRNA transfection reagents as described above.
Bankier and Bulgaria's Petya Nedelcheva are seeded fourth together in the women's doubles, with Bankier and Blair the fourth seeds in mixed doubles.
He and his friends laugh, sit around and talk and snack away on sunflower seeds together.
Heat the coriander, cumin and fennel seeds together in a frying pan and toast until they start to pop, take out immediately and spread on a tray to cool quickly.
The respective AFM topographical image of the CVD ZnO seed together with the measured line profiles is shown in Figure 1d.
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