Sentence examples for seeded media from inspiring English sources

When conditioned media were used, an equivalent number of HEK293 cells or HEK293 cells expressing Wnt3A were seeded, media were conditioned for three to four days, collected, spun at 300g for 5 minutes and filtered through a 0.45 µM filter.

After cells had seeded, media was removed and replaced with media containing 10% of human serum from 11 CR and 11 WD individuals was added in triplicate to the plates for 48hs.

Seeded media harbor considerable amounts of viable Brucella organisms, and routine bacteriologic procedures such as preparing, centrifuging, and vigorous agitation (vortexing) of bacterial suspensions, performing subcultures and biochemical testing, and particularly the catalase test, may create dangerous aerosols and the potential for accidental spillage (14).

Approximately 24 h after seeding, media was changed to RPMI without arginine (Sigma Aldrich, Madison, WI, USA) or phenol red-free RPMI-1640 contargininerGibcoe (Gibco).

The seeding media of the top two lanes was gently aspirated and the attached cells rinsed with 200 μL phenol red free DMEM (Invitrogen).

Cells of interest were washed with media, seeded in fresh media into 96-well plates at a density of 50 × 10 cells/well, treated with appropriate agents and incubated at 37 °C with 5% CO2.

The reactors were seeded with media that had developed mature anaerobic biofilm prior to the start of this study.

Cells were seeded in media without serum in the presence of 2 mM hydroxyurea, a pharmacological inhibitor of cellular ribonucleoside reductase to arrest the cell cycle in G1/S phase [35].

Cells were seeded in media with no antibiotics.

Twenty-mL aliquots of the seeded agar media were poured into 9-cm Petri plates.

Briefly, 10 cells/well were seeded with media (100  μL) in 96-well dishes in 10% FBS and incubated for 8 days.