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Since the NCAA Tournament had gone to a seeded format in 1985, no point guard had won any of the four major player of the year awards without leading his team to a #1 seed.
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Cells were seeded in the same format as for cell viability studies.
Cells were seeded in the same format as for the 7-day time course.
Cells were seeded in 48 well format, and transfected with siCntrl, siAKAP13 or siAKAP95.
For the gene overexpression analysis 0.25 × 10 S2 cells were seeded into 24-well format ∼16 hr before transfection.
Cells growing exponentially around 60 75% confluence were counted and seeded into 96-well plate format (Greiner Bio-One, Monroe, NC) at a density of 25,000 cells in 100 μL culture media per well.
For 384-well plate format, cells were seeded at 2×103 cells per well in white-bottom plates.
To assess effects of CLR01 and CLR03 on the ability of the vector to transduce cells, the vector was titrated with 0 100 µM CLR01 or CLR03 and incubated 10 min at 37°C in 50 mM HEPES, 150 mM NaCl, pH 7.4. 1 day prior to infection, 10 A549 cells per well were seeded in a 24-well format.
It's a straight knockout, devoid of fiddly format changes, with enough seeded players to add structure without stifling a strong outsider's path to glory.
Jurkat cells were seeded on a 24-well plate format and treated with the IC50 concentration of experimental compounds for 6 h.
Validation of putative MREs was performed using the dual luciferase reporter gene assay (Promega) in a 96-well format, with 4x10 cells seeded per well.
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