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We accessed the SEED metabolic sub-system page for biosynthesis and metabolism for each of these B-vitamins using the seed viewer.
MG-RAST requires an E-value cutoff under the SEED viewer (which we selected the highest).
A full diagram of this system is available through the SEED viewer at (http://seed-viewer.theseed.org/seedviewer.cgi).org/seedviewer.cgi
The syntenic core genome of Cronobacter was determined using the SEED viewer sequence based comparative genomics tool.
In this study, genome to genome comparisons were performed primarily with the SEED viewer [ 48], which utilizes bi-directional protein-protein BLAST (blastp) sequence comparison of translated ORFs.
Four genomes not present in Genbank were screened with the blast tool of the SEED Viewer v2.0 web-interface [ 73]: draft genomes of the present Xf strain LMG 25863, two X. arboricola strains LMG 19145 and LMG 19146 [ 39], and the X. arboricola pv.
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Protein-coding genes (PEG) were predicted using The RAST Server (Rapid Annotations using Subsystems Technology ; http://rast.nmpdr.org/) [ 28] and analyzed with the SEED- Viewer (http://www.theseed.org/wiki/Main_Page) [ 29].
After the annotation is complete the user can choose to download the annotated genome in a variety of export formats (e.g. GenBank, FASTA, GFF3, Excel) or browse the genome in the comparative environment of the SEED-Viewer without having the data actually installed in the SEED.
For that, we turned to the SEED-viewer within RAST.
The RAST server provided a fully automated annotation of the genome that was viewable by the SEED-viewer [22].
The SEED-Viewer environment presents the user with a variety of options for the immediate analysis of the annotated genome.
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