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Seed to seed variations were observed occasionally, even between seeds from a single harvest from an individual plant.
The seeding algorithms wihch allow some mismatches in the seeds, the BLAT seeding algorithm and the Vector seeding algorithm, is originally proposed to increase sensitivity intentionally by generating multiple seed variations from a seed.
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All simulations produced the same results (modulo random seed variation and precision loss during floating point arithmetic).
To focus on measuring only the simulation time, we disabled all output except the final species counts, and repeated each experiment 5-fold to account for initial random seed variation and possible effects of other processes executing on the system.
The PPB method also shows the presence of seed-to-seed variations with respect to seed coat iron content in this genotype (Fig. 2a).
The PPB method is ideal for the detection of seed-to-seed variations, and to determine the sites of iron accumulation in a cost-effective way.
In addition, we suggest that the PPB technique is ideal to study iron distribution in legume seeds during plant breeding and to detect seed-to-seed variations.
The seeding variation as measured by the fluorescence intensity of each well before transfection was less than 10%.
As the expression profile was analysed on bulks of five to ten seeds, variation in transgene copy number and the presence of seeds that have segregated to the wild-type may mask the silencing effect.
To take into account the variation of fluorescent intensity due to unequal initial number of cells in each well, % seeding variation was first calculated by (1) and then used to adjust the fluorescent intensity obtained from each well by (2).
A secreted form of 2G12 was expressed in the endosperm of the elite maize cultivar M37W and the best-performing line was passed through to a dedifferentiation-regeneration cycle, producing seeds yielding more than 100 μg of the antibody per gram DW and eliminating most of the seed-to-seed variation [ 76].
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