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Seed training sets contain proteins whose subcellular localization has been experimentally confirmed.
The current negative seed training set contains 400 known non-ApicoTPs extracted from ApiLoc.
Proteins localizing to the apicoplast as noted by ApiLoc are placed in the positive seed training set.
ApicoAP-CS can automatically parse ApiLoc versions to extract the information necessary to prepare seed training sets.
These proteins should also qualify to be either in the positive or the negative seed training set according to the criteria explained earlier.
Proteins localizing elsewhere as noted by ApiLoc, excluding the ones with dual localization incidents as mentioned previously, are placed in the negative seed training set.
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Founded in 2002, SEED trains teachers to design, plant and nurture a garden according to permaculture principles, which encourage a sustainable approach to agriculture modeled on the ecosystem.
Next, examples for the use of model-based concepts for the design of experiments, feeding strategies, seed train layout, and control strategies, including simulation tools supporting biotechnological training and education, are shown.
Shake flasks were then pooled and used to inoculate the first seed train bioreactor (Seed Train Bioreactor Phase) for further culture expansion (Step 2).
The seed train bioreactors were operated in batch mode, with agitation, pressure, temperature, pH and dissolved oxygen controlled during the process.
The most common method for separation, both during expansion seed trains and final product harvest stages, is to pellet the cells by dead-end (batch) centrifugation and then to resuspend the cells into a specified medium.
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