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Moreover, there were cases in which seed score was high but the phylogenetic tree appeared to support orthology.
For the homology search with and without subsequence clustering, we used a seed score threshold of Tseed = 39.
Even within PHRINGE, while numerical values (low seed score) could suggest orthology, the phylogenetic tree did not support such relationship.
The seed generating step picks the highest ranking node v, and selects a set of k - 1 neighbors of v in G S that maximize the seed score.
(2) s c o r e v i = d e g v i * d e n s i t y G v i The seed score function takes both degree centrality and neighbourhood graph density into consideration for prioritising the proteins for seeds.
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For nirS data, the best efficient discriminative seed was 14-weight spaced seed scored with 0.1697.
The highest discriminative seed was 18-weight transition-constrained seed scored with 0.8857.
In this paper, we rank proteins by their seed scores and select a fraction, denoted as λ, to be expanded into cores.
Proteins with higher seed scores are therefore more likely to be in complex cores and should be subsequently expanded to form cores and corresponding complexes.
Then p-values were re-calculated as more dissimilar words to the PWM were considered as motif instances (using the same hypergeometric scoring function as was used for the seed scoring) thus identifying the similarity threshold that led to the lowest possible p-value.
Instead, their win over No. 4 Michigan became a footnote on a day when two No. 15 seeds scored upsets.
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