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The study was designed to investigate the antihypertensive effect of total flavones extracted from seed residues of Hippophae rhamnoides L. (TFH-SR) and its underlying mechanism in chronic sucrose-fed rats by evaluating its ability to regulate insulin and angiotensin ∥ levels.
The defatted tea seed residues contain 11% to 17% saponin, which is usually used for detergents or organic fertilizers with low economic value [ 31].
Five-day-old uniform seedlings without seed residues were then carefully transferred to 1-L plastic pots (one plant per pot) filled with nutrient solution containing P1 or P50, with pH adjusted to 5.8 ± 0.2.
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The seeds are also used as bird feed, and the seed residue after oil extraction is used for fodder.
The seed residue contained a polyphenol-rich fraction that could be extracted by solvent extraction and used in other products.
The seed residue after extraction was also analyzed for polyphenol and cyanogen content and was examined by SEM to establish the effects of extraction on residual seed structure.
Fermentation of hydrolysis product obtained from 2%, 4% and 6% HCl treatments (carried out using Saccharomyces cerevisiae) gave 67.52, 74.98 and 88.62 g ethanol kg−1 dry seed residue, respectively, corresponding to ∼31.45%, 34.92% and 41.28% of theoretical ethanol (214 g kg−1) formation, calculated based on ethanol produced per gram of carbohydrate in the seed residue.
A low DF diet (LF; 17% DF) based on wheat and barley and two generic high DF diets (HF1, high in soluble DF and HF2, high in insoluble DF; ∼ 41% DF) where the cereals were substituted with co-products (sugar beet pulp, potato pulp, pectin residue, pea hull, brewer's spent grain and seed residue (ray grass)) from the vegetable food and agro industries.
Among the reaction variables considered, acid concentration and temperature showed a positive effect on glucose release from the biomass with HCl the best catalyst compared to H2SO4 and H3PO4 showing highest glucose formation (173.4 g kg−1 seed residue) at 100 °C with 6% w/w HCl concentration.
The findings provide a solid foundation for future identification and characterization of seed storage lipids and protein and, ultimately, of genetic engineering to introduce desirable characteristics: for example, to improve the protein nutrient value of seed residue after lipid is extracted for biodiesel production.
After winter wheat seeding, residue cover declined for all tillage treatments, but was still highest at 40% residue cover under NT.
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