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We used this vector because it contains a RFP seed marker which allows for easy identification of plants which contain the reporter.
Primary transformants were identified using the RFP seed marker contained in the pFAST-R07 construct and were allowed to self-fertilize to generate homozygous lines.
The obtained testcross phenotypes were regressed on the corresponding landrace seed marker genotypes using ridge regression to train a genomic prediction equation [ 27, 35, 36].
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The selected genes behaved as good seed markers, while for mesocarp their reliability appeared to be dependent upon developmental and ripening traits.
Seed markers were a LTP1 (lipid transfer protein), a PR (pathogenesis-related) protein, a prunin and LEA (Late Embryogenesis Abundant) protein, for S1, S2, S3 and S4, respectively.
Melamed-Bessudo et al. (2005) introduced transgenic A. thaliana lines carrying two fluorescent seed markers that the authors used to estimate meiotic recombination frequencies (MRFs) between the two marker loci in diploid Arabidopsis plants.
As regards seed markers, ctg3431, coding for a LTP, clearly marked the S1 stage for both Fantasia and slr, while in Springcrest its expression decreased only at S3 stage.
In the latter case, data indicate that, while seed markers represent reliable tools in all the tested varieties, in the case of the mesocarp the different developmental and ripening traits of the various genotypes somewhat affect the expression of marker genes, consistently with their putative functions and cv characteristics.
Enter/Quit a seed point marker input mode by pressing the 's' key.
Based on the allele frequencies estimated from our large sample of rice germplasm, researchers can select subsets of verified SNPs that segregate in specific subsets of genetic materials as the basis for targeted assays designed for QTL mapping, pedigree analysis, tests of hybrid or varietal seed purity, marker-assisted selection, etc.
In this study, GWA scans have allowed us to identify soybean seed composition markers with <1 Mbp resolution.
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