Infections were performed just after seeding; in the case of infections with smFAIM-L, neurons were also reinfected 3 days before treatments.
A plant needs to flower in order to set seed, and in the case of many crop plants, including legumes and cereals, the seed serves as a main source of food in large parts of the world.
The seeder worked satisfactorily at suction pressures of 4.91 and 3.92 kPa and nozzle diameters of 0.46 and 0.49 mm to achieve more than 90% single seed sowing in the case of capsicum and tomato, respectively.
For the research-grade autoclave with an internal diameter of 2.22 cm, the constraint for the GaN growth is found to be the growth kinetics and the total area of seed surfaces in the case of baffle opening of 10% (including the central opening of 5% and ring opening of 5%).
Examination of the gross morphologies of the dusts revealed that, except for corn, each dust contained either husk or pericarp (seed coat in the case of flax) fragments in addition to respirable particles.
Instead, when we did find non-AG ants at AG seed baits, they appeared to ignore the seeds, or in the case of C. sericeiventris, to be alarmed by them an outcome that has also been reported previously [5].
Neurons were transfected between 7 to 12 days after seeding or, in the case of the cell lines, 1 day after seeding using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer's protocol.
However, while all combinations of Y and/or Yh are lethal in Papaya, in Vitis HH genotypes do strive and set seeds, as in the case of certain domesticated grapevines such as Chardonnay, Muscat de Hambourg, Riesling or Cardinal (Truel pers. comm., Vassal INRA), which produce 100% hermaphrodite progenies.
However, the method cannot be used to detect iron in darkly pigmented seed coats, as in the case of DOR364.
