This was further confirmed by estimating the level of sulphate in the seed compartment (sulphate content × seed mass per plant) and per seed (sulphate content × 1-seed weight), which increased by ~60% in s ultr4;1 seeds compared to wild-type (see Additional file 3).
For the other anions measured, only nitrate content was found to increase in s ultr4;1 seeds but nitrate levels in the seed compartment or per seed were not significantly different compared to wild-type (see Additional file 3), suggesting s ultr4;1 is particularly affected in seed sulphate content.
There, unspecialized cells called stem cells divide to make more of themselves or specialize into specific plant parts, like carpels, the flower organs that make seed compartments.
In addition, differential spatial expression of DUF642 genes among various seed compartments during germination has been reported in Arabidopsis thaliana.
For instance in olive (Olea europaea), two FAD2 genes (OeFAD2-1 and OeFAD2-2) arexpresseded both in two seed compartments, seed coat and embryo.
The other two genes, CaFAD2-C1 and CaFAD2-C2, might be responsible to change fatty acid contents in other seed compartments (e.g. seed coat and endosperm).
In total, we obtained 88 M reads from 10 SOLiD libraries (9 stages of whole seeds and flower buds) and 295 M reads from 17 SBS libraries (dissected seed compartments).
For example, if users click horizontal bars below 'GSE7511'Expressionion data from soybean seed compartments with embryos at the heart stage'), it will show gene Glyma15g34770's (represented by probe set 'GmaAffx.88235.1.S1_at') expression profile in different tissues of soybean seed.
For seed compartments, fresh seeds were manually dissected into radical, hypocotyl, cotyledon, embryo, endosperm and seed coat in RNAlater (Life Technologies) and kept at 4°C for a few days for the solution to penetrate into the seeds and then stored in −20°C.
In angiosperms, the endosperm plays a crucial placenta-like role in that not only is it necessary for nurturing the embryo, but also regulating embryogenesis through complicated genetic and epigenetic interactions with other seed compartments and is the primary tissue in which genomic imprinting occurs.
Cells were seeded into compartments of 8-well Lab-Tek® II chamber slide (Nalge Nunc International, Naperville, IL), allowed to attach onto the slide and deprived of serum for 18 h prior to the incubation with 0.2 μM HNTMB for 24 h.
