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These results imply that TT2 could be more closely related to TTG1 activity in affecting seed characters.
These observations suggest that both TTG1 and its interacting partner TT2 control seed characters genetically downstream of SK11 and SK12.
However, g4HA-TTG1S215E and g4HA-TTG1S215A had opposite effects on seed characters of ttg1-13 (Fig. 5b, c).
Since SK11 and SK12 interacted with TTG1 and affected seed characters upstream of TTG1, we reasoned that TTG1 could serve as a substrate of SK11 and SK12.
This is in contrast to the opposite roles of g4HA-TTG1S215E and g4HA-TTG1S215A in specifically affecting ttg1-13 seed characters (Fig. 5b, c).
To find out how interaction between TTG1 and TT2 affects downstream molecules to control seed characters, we selected GL2 as a potential target of TTG1 and TT2 based on the following two reasons.
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All seed quality characters investigated were monogenic and could be identified with genes previously identified in pea.
These genes control traits such as pod dehiscence, seed dormancy, seed size and other seed quality characters, stem height, root mass, and harvest index.
Investigating seed quality characters from Jatropha germplasm, demonstrated a large variation in seed and oil traits related to different geographic locations or different environmental conditions [ 65, 66].
For example, 16% of DPRK improved varieties in this study had a white awn, which is generally selected against in modern breeding programs (Sweeney and McCouch [2007]), but was a common seed character in 80% of native or landrace Korean varieties collected during 1911 1923 (Choi [2005]).
We define seed dormancy here as a seed character that prevents germination, even if suitable germination conditions prevail.
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