Conversely, however, the deletion of over 1,000 CNEs in mutant strains of mice was not seen to cause any detectable deleterious effects in terms of phenotype, gene expression and fitness [ 7].
In ITC experiments, we saw no detectable binding when we added IP6 to a PH-TH module with mutations at both the canonical and the peripheral sites, which confirms that the residual binding of IP6 to the IP4-binding deficient mutant is due to binding at the peripheral binding site.
In several other lines, widespread centralisation of myocyte nuclei was seen without detectable muscle weakness, high serum creatine kinase levels or prominent inflammatory infiltrates, demonstrating a muscle phenotype that was not revealed at this stage of the disease by physiological assays.
In ITC experiments, we saw no detectable binding when we added IP6 to a PH-TH module with mutations at both the canonical and the peripheral sites, further confirming that the residual binding of IP6 to the IP4-binding deficient mutant is at the peripheral binding site.
First, they are made independently by two programs based on different principles; second, and more strikingly, the helices are predicted in the same region of αI in the absence of any detectable sequence similarity (see Figure 5 ).
Based on multi-enzyme GC-MS assays, we will only see a GC-detectable terpene product from the alternative pathway if RcMPD is able to decarboxylate MVAP to IP, the latter of which is a substrate for IPK to make IPP.
For serum 25-1, however, the unbound fraction was depleted of any detectable SRP54N reactive antibodies (see Figure 2b, lane 14) and showed no reaction with either SRP54G or SRP54M.
In parallel experiments in HUVECs, VEGF-A165 induced strong ERK1/2 and Akt activation, whereas neither PDGF-AA nor PDGF-BB had any detectable effect in HUVECs (see Supplementary Figure S2C).
In contrast, after immunization with adenovirus-HuD we saw almost no detectable proliferation of HuD p321-specific CD8+ T cells directly ex vivo.
In the CAF01 group, peak parasitemia occurred around day 10 post challenge with a mean of 35% ± 2.5 after which a clearance of parasites from the circulation was seen with no detectable infected erythrocytes from day 15 and onwards (Fig. 5D).
