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In order to characterise Muc5ac and Muc5b, four tracheal wash samples from horses showing signs of mucus hypersecretion were pooled, solubilised in 4M guanidinium chloride and analysed by caesium chloride density gradient centrifugation (buoyant density), anion exchange chromatography (charge distribution) and rate-zonal sedimentation (size distribution).
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Sedimentation measurements and observations could be lead as well, using several sediment size distributions.
Quartz sand was used after grinding and size-fractionation by gravity sedimentation (particle size, determined by the gravimetric method: <2 µm).
Regression analysis shows that adsorption, sedimentation, and size are all highly significant independent predictors of delivery enhancement.
Each combination of flocculation and shear preparation was carried out in duplicate and tested for sedimentation and size distribution properties as described below (n = 2).
Sediment cores were also collected along a transect crossing through the meander neck in order to evaluate changes in grain size, sedimentation rates during the cutoff event, and gain further insight into the velocity of migration of meander bends.
After 7 days storage, the nanofluid showed no sedimentation, unchanged particle size, and zeta potential.
Amines were revealed to be a key chemical group for adsorption, while delivery was shown to be quantitatively predictable by molecular adsorption, GO sedimentation, and GO size.
The lumped properties as represented by ultra scale-down centrifugation results were correlated with the basic properties affecting sedimentation including particle size distribution, suspension viscosity, and solids volume fraction.
For a power of 80% at 0.05 precision, and an expected increase in positivity from 27%to32%2% with SCA and bleach sedimentation, the sample size required was 543 patients (McNemar test).
The regression coefficients for adsorption (b1), sedimentation (b2), and size (b3) are 1.0 × 10 2, −1.4 × 10 3, and −7.2 × 10 3, respectively, when the variables are expressed in units of Gd/10C, AUC, and nm.
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