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In the absence of Pep2, TreS sediments at a sedimentation coefficient of 11 S, with a much smaller maximum at 16 S.
In density gradients, particles sediment according to their sedimentation coefficient (s).
By analytical ultracentrifugation analysis, recombinant HP-NAP was sedimented as a single peak with a sedimentation coefficient of 9.8 S.
(Arrays missing H2A sedimented slightly slower than WT arrays with a sedimentation coefficient of 22.7 S).
The sedimentation coefficient distributions were obtained using the c(s) method of SEDFIT ver. 15.01b61.
(Sedimentation coefficient). A standardized value, describing migration of molecules/particles under a centrifugal force.
Sedimentation velocity gave a sedimentation coefficient of 4.49(±0.11) S.
Using this model the sedimentation coefficient of an individual macromolecule is recalculated into the sedimentation coefficient of semidilute solution.
c, Continuous sedimentation coefficient (c(s)) distributions for the TfR1 Tf complex (2.5 μM complex) in the presence of increasing concentrations of PvRBP2b(161–969) (0.25–16 μM).
Data analysis was carried out using SEDFIT40, which employs the continuous c(s) conformational change model based on the Lamm equation, to determine the sedimentation coefficient distribution.
The configuration and surface charge properties of the ternary complex suggest that this particle may display non-ideal sedimentation, possibly contributing to the observed shift in sedimentation coefficient.
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