It is difficult to draw clear conclusions from these studies, partly because of disregard of possible changes in breathing pattern, e.g. due to sedation, test gas used [19], [20] or sighs [21].
There are also fees for sedation, blood tests and drugs that can add up to £2,335.
While the animals were under sedation, ABR testing was performed in response to 4-, 8-, 16-, and 32-kHz tone bursts.
Two 10 mL vials of blood (20 mL total) were drawn from peripheral vessels under sedation and tested for bacteria, complete blood count and elemental analysis pre- and postoperatively, at weeks 1, 2, 4, and prior to sacrifice.
Degree of sedation (chi-squared test), heart rate, and blood pressure (t-test) were similar in both groups at each time point.
Acepromazine was used to determine the influence of sedation on the testing results.
Considering the overall quality of the different protocols tested, sedation using alphaxalone shows to be the most optimal as we observed no (excessive) salivation, apnoea, involuntary muscle movement, or vomiting for this protocol.
The procedure codes assigned the procedures undertaken during each visit, (e.g. flexion test, sedation) and also determined if the visit was a first visit or a follow up visit to the clinic.
PR (beats/min), MAP (mm Hg), body temperature (°C), SpO2 (per cent), and RR (per min) in the tested sedation protocols during the first 9 min of the immobilisation period, with 3 min interval.
sedation awakening trial.
