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However, many cells are part of a sectorized deployment, which implies that the cell towers of these sector cells have the same location but their antenna patterns are aligned differently.
Twelve mm path length double sector cells were used for all samples.
Samples containing 2 µM oligonucleotide, 10 µM monomeric NsrR in 50 mM Tris pH 8.0, 100 mM NaCl, 1 mM dithiothreitol were loaded into charcoal-filled Epon double sector cells fitted with quartz windows.
Sedimentation velocity experiments were run at 30,000 RPM (22°C) on an Optima XLI (Beckman-Coulter, Fullerton, CA), with a 4-hole An60Ti rotor using double sector cells with charcoal-filled Epon centerpieces (path length 1.2 cm) and sapphire windows.
Epon charcoal double sector cells (12 mm) assembled with quartz windows were loaded with ∼440 μL reference solution and ∼430 μL sample solution, radially calibrated, and thermally equilibrated at 20 °C for 1.5 2.0 h prior to initiating the experiment.
AUC (analytical ultracentrifugation) equilibrium measurements were carried out on an Optima XL-A (Beckman Coulter) instrument equipped with absorbance optics using six sector cells and scanning at 280 nm.
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where dleft is the leftmost direction of a circular sector cell form and dright is the rightmost one.
Given our understanding of circular sector cell configurations, we developed an iterative data cleansing and compensation method.
The irregular fingerprints in the line sections are detected by finding the leftmost and rightmost directions of a circular sector cell form.
The value for the load can be the maximum acceptable load (MAL) per sector (cell) or it can be the predicted load (PL) per sector during the busy hour.
The slit window was mounted together with a normal window on a single sector cell such that the slit was facing the inside surface of the centerpiece.
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