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This swallows up what was left of the business department – after large sections were transferred to other ministries – along with energy and climate change, and brought criticism that May was not treating environmental concerns seriously enough.
Sections were transferred to the wells of a 24-well plate containing PBD PBSS + 0.1% Tween-20 and 0.5% Triton-X-100), which was then replaced with blocking solution (PBD + 1% BSA) for 1 h at RT.
Ultrathin sections were transferred to the 300 mesh copper grid and stained with 5% uranyl acetate.
The 8 um-thick sections were transferred onto poly-L-Lys-coated glass slides, deparaffinized in xylene, and dehydrated through an ethanol series.
Its northern- and easternmost sections were transferred to other highways in the 1930s.
Sections were transferred to Formvar-coated nickel grids (150 mesh).
Sections were transferred onto Superfrost Plus coated slides (Fisher Scientific, USA) and stored at −80°C.
After the TBS wash, sections were transferred onto slides, mounted and imaged as described above.
Hand-cut cross sections were transferred into curved slides, washed thoroughly with deionized water and analyzed by CLSM (see below).
After rinsing the sections were transferred to biotinylated anti-rabbit IgG (Vector, USA; 1∶200 for 2 h).
Sections were transferred to PB at 4°C (with 0.01% sodium azide if stored for more than 2 days).
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