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Tissue sections were sliced into 4 μm-thick sections using a Reichert Jung Ultracut E ultra-microtome (Scotia, NY, USA), affixed onto clean slides, and placed at 60°C until dry.
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The paraffin blocks were sliced into sections that were then stained with hematoxylin and eosin.
Paraffin-embedded tissues were sliced into sections with 5 μm thickness.
Frozen gastrocnemius muscle samples were sliced into sections (8-μm thick) and mounted on silane-coated glass slides.
Shoot apices containing leaf primordia were embedded in 5% agar and then agarose blocks were sliced into sections with a vibratome.
Similarly, lymph nodes were sliced into thin sections (about 2mm thick) and inspected for the presence of visible lesions.
Lymph node tissues were sliced into thin sections (1 to 2 mm thick) and examined for the presence of visible lesions.
Embedded tissues were sliced into ultrathin sections and stained with uranyl acetate and lead citrate.
In all, the 18-cm long gel strips were sliced into 26 sections.
After the leaching, the columns were sliced into 5-cm sections for the analysis of water-extractable P (WEP).
Then the frozen tissues were sliced into µm thin sections.
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