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Whole tumor sections were quantified for specific LOX-staining intensities.
For reasons of practicality, five stepped sections were quantified for all markers within Studies A and B. Exploration of the data for all transformed markers revealed them to be log-normally distributed.
A total of nine sections were quantified for each mouse strain and the mean value and SEM was calculated and compared with the wild-type C57Bl/6 mice in percentage of control.
Five artery and three kidney sections were quantified for intensity and area of the immune-reactive regions using the AxioVision (rel. 4.8) software (Zeiss, Jena, Germany).
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GFP fluorescence in each z section was quantified for the whole cell and for a central region containing the kinetochores and most of the spindle.
For immunohistochemistry, sections were quantified using ImagePro Plus version 5.0 (Media Cybernetics, Bethesda, MD, USA).
For each animal, at least three sections were quantified to yield an average value, and at least three different animals were analyzed for each experimental situation.
For semiquantitative analysis, two sections were quantified.
Immunostained sections were quantified by three independent observers.
Immunofluorescent sections were quantified using Nikon Elements imaging software (Melville, NY).
Positive cell counts per section area were quantified for each of the markers using whole-slide scans.
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