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Free-floating sections were preserved in antifreeze solution until immunohistochemistry was performed.
Sections were preserved in PBS with 0.01% sodium azide.
(i) For standard and real-time PCR/RT-PCR analysis on whole spinal cord sections, the extracted spinal cord sections were preserved in RNAlater (Ambion), as described by the manufacturers' instructions, until further processing (see below).
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Sections of the sample were preserved in a methylmethacralate mold, sliced via a hard microtome, and polished systematically.
Afterwards, brain tissue was cut in a cryostat (−22°C, Microm HM 505 N) into coronal sections at 40 μm thick, which were preserved in a 30% sucrose and 0.1% timerosal in PBS at 4°C [ 26].
The expression of mesothelioma specific immunohistochemical markers in primary cell cultures were preserved in the tumor sections from mouse xenografts.
Sections of the protein (& corresponding sections of the gene) that were essential to IF formation were preserved in all duplicates.
In short, representative tissue blocks from the upper and lower lobes were preserved in 4% formaldehyde, sectioned and stained with haematoxylin and eosin.
Cross-sections of both ventricles and the interventricular septum were preserved in RNAlater (Ambion Inc., Austin, TX) for oligonucleotide array analysis and frozen at -70°C until processing.
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Multiple organs were preserved in 10% neutral phosphate buffered formalin, and processed for histologic sectioning.
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