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Tissue sections were observed for relative degree of inflammatory lesions.
8 sections were observed for each marker, with a section evaluation interval of 6.
The sections were observed for EGFP fluorescence using a Olympus BX60 microscope under UV illumination.
As shown in Figure 3, HE staining and sirius red staining of liver sections were observed for histological changes.
The sections were observed for OA-like features such as surface discontinuity, loss of proteoglycans, disorientation of chondrocytes, subchondral bone sclerosis and presence of cysts and osteophytes.
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Stained sections were observed under a microscope for glandular structure and leukocyte infiltration determination.
H&E-stained sections were observed under a microscope for glandular structure and leukocyte infiltration.
Stained sections were observed at × 100 magnifications for glandular structure and leukocyte infiltration.
For vascular density, sections were observed with a Leitz DMRB microscope (Leica, Wetzlar, Germany) equipped for fluorescent microscopy.
In brief, the cytokeratin-stained whole tissue sections were observed at low magnification (× 10) for identifying the tumour margin area with the highest density of tumour budding.
On PI 4,5 P2 membranes, by increasing membrane tension, the protein fluorescence signals on the tether cross sections were observed to increase, while the fluorescence intensities for the lipid channel were decreasing.
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