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Stained tissue sections were observed by bright field microscopy by an observer blinded to the genotype.
More than five sections were observed, and representative images were shown.
More than five sections were observed, and representative images are shown.
Counterstained (uranyl acetate and lead citrate) ultrathin sections were observed under an electron microscope (Philips 400 S, Eindhoven, The Netherlands).
Sections were observed under an Axio Scope A1 microscope (ZEISS) equipped with an AxioCam ERc 5 s camera (ZEISS) and analyzed using the ZEN2 software (ZEISS).
Thin sections were observed with a polarizing microscope at magnifications of ×12.5 to ×400, using plain polarized (PPL), cross-polarised (XPL), and oblique incident light (OIL).
The tissue sections were observed with CLSFM.
The sections were observed under a Leica DMLB microscope.
The stained sections were observed under a light microscope.
Finally, the sections were observed in a JEM1230 transmission electron microscope (JEOL Ltd., Tokyo, Japan).
After staining, the sections were observed under a transmission electron microscope.
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