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Some sections were left almost unrecognizable.
Kidney sections were left unstained.
Sections were left at 4°C in 25% sucrose in PBS for 24 h to dehydrate.
Sections were left 20 min at room temperature after each microwave treatment.
For double labelling, stained sections were left for 24 h at room temperature with N52, PGP or CGRP as described above.
The sections were left to cool prior to reduction with 10 mM dithiotreitol (DTT) and alkylation with 25 mM iodoacetamide in 0.1 M Tris-HCl pH 8; each treatment was applied for 30 minutes.
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Some sections are left only partly excavated so that visitors can see the magnitude of the job that entails carefully digging out the broken fragments of the fully dressed soldiers.
To avoid simulating excess caesarean section rates in all institutions (particularly at ISSSTE) [ 22], the number of daily procedures including both non-surgical procedures including vaginal deliveries and surgical procedures including caesarean sections was left at one per doctor per day.
The last section is left for the proof of the main result.
For the smooth tube and spirally fluted tube, the materials are all stainless steel, the equivalent diameters are the same, the lengths are all 1200 mm, the test sections are all the middle section 1000 mm of the tube, and 100 mm section is left at each end of the tube in order to avoid the entrance effect.
The middle section is left in an intermediate position for the warmer nine months of the year so that vehicle traffic can use the lower deck of the lift span and pleasure craft can pass under the bridge.
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