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Sections were incubated with LSAB2 (Dakocytomation).
Control sections were incubated without primary antibody.
Sections were incubated subsequently with 1% horse serum in TBS.
Sections were incubated overnight with primary antibodies at 4° Celsius.
The negative control sections were incubated with 0.01 M PBS.
Control sections were incubated in the absence of saccharopine.
Negative control sections were incubated with secondary antibody only.
Then, sections were incubated 10 min with avidin and biotin.
The sections were incubated with anti-PCNA (BioGenex Inc).
Sections were incubated overnight.
Deparaffinized sections were incubated with primary antibodies.
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