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Tissue sections were fixed in 10% buffered formalin and embedded in paraffin.
Sections were fixed in 1 1 acetone-to-methanol and incubated with PNA FISH probes at 55 °C.
For immunofluorescence, frozen tissue sections were fixed in cold methanol for 10 minutes and air-dried.
The tissue sections were fixed in 1% paraformaldehyde for 2 h at 4 C.
Sections were fixed in 4% paraformaldehyde.
Frozen sections were fixed in 10% formamide.
Frozen sections were fixed in 10% formalin.
Sections were fixed in 4% paraformaldehyde PBS, followed by acetylation.
For immunofluorescence, 5-µm thick OCT embedded fresh frozen sections were fixed in ice-cold methanol.
The sections were fixed in methanol/acetic acid and stained in hematoxylin.
The sections were fixed in 10% buffered formaldehyde solution overnight at 4°C.
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