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The sections were examined using a FEI Tecnai spirit at 80 KV and photographed with an AMT CCD camera.
Undecalcified histological sections were examined using microradiography and fluorescence microscopy after sequential intravital polychromic labelling.
The tissue sections were examined using a confocal microscope with an UPlanSApo 20×/1.35 oil immersion objective lens (Fluoview FV1000, Olympus, Tokyo, Japan).
The stained sections were examined using TEM.
The thin sections were examined using an Axio Scope A1 microscope made by Zeiss.
Thin sections were examined using a Zeiss Axio Scope A1 stereo microscope for the identification of sandstone shapes.
At the end of the study, at 36 weeks of age, eye tissues were collected and retinal sections were examined using light microscopy and transmission electron microscopy.
Full-stained ultra-thin sections were examined using the transmission electron microscope (JEOL-JEM-100SX, Japan) with beam current = 60 μA, and high voltage of 80 kV.
After staining with hematoxylin and eosin (H&E), the sections were examined using a light microscope to determine the presence of derivatives from the three germ layers.
The sections were examined using an Olympus BX60 microscope.
Light microscopic sections were examined using a Leica DMRB brightfield microscope (objectives 1.6× to 20×).
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