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Thin sections were cut with a diamond knife and stained with uranyl acetate and lead citrate.
Serial ultrathin (70 or 100 nm) and semithin (2 μm) sections were cut with a diamond knife (Diatome).
After cryoprotection in 30% sucrose/PBS (pH 7.4), brains were frozen and serial coronal sections (60 μm sections) were cut with a microtome.
Polymerized blocks were trimmed and 70 nm ultrathin sections were cut with a diamond knife on a Leica Ultramicut EM UC7 and transferred onto 200 mesh copper grids.
Then, 3 µm-thick sections were cut with glass knives using a Reichert-Jung 2040 Autocut rotary microtome.
From the central part of the teeth, three sections were cut with a thickness of 110 μm.
Ultrathin (70 nm) sections were cut with an ultramicrotome and stained with 5% uranyl acetate and 2% lead citrate.
Serial, 3 µm-thick sections were cut with glass knives using a Reichert-Jung 2040 Autocut rotary microtome.
After polymerization for 8 h at 70°C, 1.5-μm thick sections were cut with glass knives on an EM UC6 ultramicrotome (Leica Microsystems, Wetzlar, Germany).
Serial semi-thin (1.5 μm) sections were cut with an Ultracut UC-6 (Leica), mounted onto slides and stained with 1% toluidine blue.
Ten µm sections were cut with a microtome.
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